Studi In Vitro Aktivitas Antioksidan dan Antikolesterol Fraksi Etil Asetat Buah Pedada (Sonneratia casiolaris)
DOI:
https://doi.org/10.57214/jka.v10i1.1052Keywords:
Anticholesterol, Antioxidant, Ethyl Acetate Fraction, Pearson Correlation, Sonneratia caseolarisAbstract
Pedada fruit (Sonneratia caseolaris) is a mangrove species recognized for its rich content of bioactive compounds that may provide various health benefits. This study aimed to evaluate the in vitro antioxidant and anticholesterol activities of the ethyl acetate fraction of pedada fruit and to analyze the correlation between these two biological activities. The extraction process was conducted using ethanol maceration followed by solvent–solvent fractionation to obtain the ethyl acetate fraction. Antioxidant activity was measured using the ABTS radical scavenging method, while anticholesterol activity was assessed through an in vitro cholesterol inhibition assay. The effectiveness of both activities was expressed in IC₅₀ values. The results demonstrated that the ethyl acetate fraction exhibited strong antioxidant and anticholesterol properties, with IC₅₀ values of 20.555 ppm and 9.636 ppm, respectively. Furthermore, Pearson correlation analysis showed a positive and statistically significant relationship between antioxidant and anticholesterol activities (r = 0.601; p = 0.005), indicating promising potential as a natural therapeutic agent.
References
Aini, R. N. (2023). Antioksidan ekstrak etanol dan infusa daun rambutan (Nephelium lappaceum L.) dengan metode ABTS. Duta Bangsa Surakarta.
Arifin, B., & Ibrahim, S. (2020). Structure–activity relationship of flavonoids as antioxidant. Asian Journal of Pharmaceutical and Clinical Research, 12(5), 8–13.
Arifin, H., Oktavia, S., & Chania, S. (2019). Effects of sub-acute toxicity of water fractionation of ethanol extract of bandotan leaves (Ageratum conyzoides (L.) L.) against several blood parameters of male white mice. Jurnal Farmasi Higea, 11(2), 166–174. https://doi.org/10.36387/jifi.v2i2.412
Ayunda, R. D., & Malita, S. (2024). Pemanfaatan senyawa flavonoid sebagai antioksidan pada penderita hiperkolesterolemia: Studi literatur. Jurnal Kedokteran Unram, 13(3). https://doi.org/10.29303/jk.v13i3.5388
Chen, G., Luo, J., & Yang, L. (2021). Plant phenolics as cholesterol-lowering agents: Mechanisms and applications. Food Chemistry, 343, 128528. https://doi.org/10.1016/j.foodchem.2020.128528
Das, S., & Ghosh, S. (2020). Antioxidant and hypolipidemic activity of medicinal plants. Clinical Phytoscience, 6(1), 1–12.
Fitri, A. (2021). Aktivitas antioksidan ekstrak metanol daun mangrove (Avicennia marina).
Harefa, Y. S., & Yulianti. (2023). Studi in vitro potensi antioksidan dan aktivitas antidiabetes fraksi etil asetat buah parijoto (Medinilla speciosa B.). Journal Health of Education, 4(1).
Layreinshia, B., & Atzmardina, Z. (2024). Edukasi mengenai makanan sehat untuk menanggulangi hiperkolesterolemia pada masyarakat Desa Cikupa. Syntax Literate: Jurnal Ilmiah Indonesia, 9(1), 369–373. https://doi.org/10.36418/syntax-literate.v9i1.14843
Mingle, C. E., & Newsome, A. L. (2020). An amended potassium persulfate ABTS antioxidant assay used for medicinal plant extracts revealed variable antioxidant capacity based upon plant extraction process. bioRxiv, 7, 1. https://doi.org/10.1101/2020.07.15.204065
Nguyen, Q. V., Huyen, B. T., Tran, M. D., Nguyen, M. T., Doan, M. D., Nguyen, A. D., Le, T. M., Tran, V. C., & Pham, T. N. (2022). Impact of different drying temperatures on in vitro antioxidant and antidiabetic activities and phenolic compounds of wild guava leaves collected in the Central Highland of Vietnam. Natural Product Communications, 17(4). https://doi.org/10.1177/1934578X221095349
Nusaibah, Sari, R. M., & Widianto, D. I. (2022). Pemanfaatan ekstrak daun pedada (Sonneratia caseolaris) dan bayhops (Ipomoea pes-caprae) sebagai antioksidan pada formulasi face mist. Jurnal Pengolahan Hasil Perikanan Indonesia, 25(3), 441–456. https://doi.org/10.17844/jphpi.v25i3.42563
Pham, D. C., & Nguyen, T. V. (2022). In vitro antioxidant capacity and hypolipidemic effects of mangrove plants. Journal of Herbal Medicine, 32, 100529.
Putri, D. A., et al. (2021). Aktivitas antioksidan tanaman mangrove menggunakan metode ABTS. Jurnal Farmasi Indonesia, 18(2), 120–128.
Rice-Evans, C. A., Miller, N. J., & Paganga, G. (2021). Structure–antioxidant activity relationships of flavonoids. Free Radical Biology and Medicine, 20(7), 933–956. https://doi.org/10.1016/0891-5849(95)02227-9
Takó, M., Kerekes, E. B., Zambrano, C., Kotogán, A., Papp, T., Krisch, J., & Vágvölgyi, C. (2020). Plant phenolics and phenolic-enriched extracts as antimicrobial agents against food-contaminating microorganisms. Antioxidants, 9(2), 1–20. https://doi.org/10.3390/antiox9020165
Tinggi, D. A. N. K. (2025). Tepung pedada: Solusi alami untuk diabetes (Issue July).
Wahyuni, S., Mahmuda, I. N. N., Maryati, Perdana, A. P., & Azizah, M. N. (2023). Studi komparatif in vitro aktivitas antioksidan ekstrak etanol, fraksi etil asetat dan isolat aktif kulit delima (Punica granatum L.). Biomedika, 15(1), 67–75. https://doi.org/10.23917/biomedika.v15i1.1751
Zhang, Q., Yang, W., Liu, J., Liu, H., Lv, Z., Zhang, C., Chen, D., & Jiao, Z. (2020). Identification of six flavonoids as novel cellular antioxidants and their structure–activity relationship. Oxidative Medicine and Cellular Longevity, 2020. https://doi.org/10.1155/2020/4150897
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